Steps of Glycolysis — Prelims Strategy

Prelims Strategy

To excel in NEET questions on glycolysis, a systematic approach is essential. Firstly, memorize the 10 steps in sequence, along with the substrate, product, and enzyme for each. Creating a flow chart or diagram can be highly effective.

Pay special attention to the three irreversible steps (Hexokinase/Glucokinase, PFK-1, Pyruvate Kinase) as these are frequent targets for questions on regulation. Understand the energy balance: 2 ATP consumed, 4 ATP produced, leading to a net of 2 ATP, and 2 NADH produced.

For numerical problems, always distinguish between gross and net ATP. For conceptual questions, focus on the 'why' behind each step, such as why glucose is phosphorylated (trapping, activation) or why DHAP is isomerized to GAP.

Be wary of trap options that might confuse enzyme names, reverse reactions, or misrepresent ATP/NADH counts. Practice identifying the specific step where ATP is consumed or produced, or where NADH is generated.

Also, be prepared for questions on the fate of pyruvate in different conditions (aerobic vs. anaerobic) and the purpose of fermentation.