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CRISPR Technology — Prelims Questions

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Version 1Updated 10 Mar 2026

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Q1medium

Which of the following statements regarding CRISPR-Cas9 technology is/are correct? 1. The Cas9 enzyme is guided to its target DNA by a specific RNA molecule. 2. The Protospacer Adjacent Motif (PAM) sequence is crucial for Cas9 binding and cleavage. 3. Non-Homologous End Joining (NHEJ) is a precise repair pathway used for gene correction. 4. Germline gene editing using CRISPR is currently permitted for therapeutic purposes in India.

Q2medium

Consider the following applications of CRISPR technology: 1. Developing disease-resistant crop varieties. 2. Diagnosing infectious diseases rapidly at point-of-care. 3. Correcting single-point mutations responsible for genetic disorders. 4. Engineering mosquitoes to control vector-borne diseases through gene drives. Which of the above applications are currently being explored or implemented using CRISPR technology?