Biotechnology Principles — Prelims Questions

Which of the following is NOT a necessary component for a cloning vector?

Which enzyme is responsible for joining the foreign DNA fragment with the vector DNA?

Which of the following statements about restriction enzymes is INCORRECT?

In the process of rDNA technology, after the insertion of recombinant DNA into a host cell, how are the transformed cells typically selected?

A plasmid vector pBR322 has two antibiotic resistance genes: ampicillin (AmpR) and tetracycline (TetR). If a foreign gene is inserted at the BamHI site, which is located within the TetR gene, what will be the phenotype of the recombinant transformants?

Which of the following is a heat-stable DNA polymerase used in PCR?